The nitrogen atom in the following cyclic compounds can be removed as trimethylamine by successive Hoffmann eliminations (involving exhaustive methylation followed by heating with $\mathrm{AgOH}$). The amine which will require a greater number of Hoffmann eliminations is

The product of the following reaction is

The product $\left(\mathrm{A}\right)$ of the above reaction is:

The product $\mathrm{B}$ of the following sequence of reactions is 

${\mathrm{C}}_6{\mathrm{H}}_5{\mathrm{CONH}}_2\underset{\mathrm{NaOH}}{\overset{{\mathrm{Br}}_2}{\to }}\mathrm{A}\underset{0-5°\mathrm{C}}{\overset{{\mathrm{NaNO}}_2/\mathrm{HCl}}{\to }}\mathrm{B}$

A mixture of three amino acids glycine (Giy), Iysine (Lys) and glutamic acid (Glu) was separated by an anion exchange chromatography process. The order of elution of amino acids from the column is_____.