PCR or polymerase chain reaction is a technique used for the amplification of a small segment of DNA. It was discovered by Kary B Mullis in 1983. The reaction is carried out in a thermal cycler having a thermal cycling programme. It includes three steps: denaturation, primer annealing and extension. The target gene is put along with other substrates in the thermocycler. The DNA fragment becomes double at the end of the first cycle and quadruples after the second cycle and so on. In this process, no host cell is used as the supporting system.

PCR stands for Polymerase Chain Reaction, which is a technique commonly used in molecular biology and biotechnology labs to create millions of copies of a small segment of DNA. This teсhnique wаs develорed in by Kаry Mullis, аn Аmeriсаn biосhemist in the year 1983. The majority of PCR methods rely on thermal cycling in which reactants are exposed to repeated heating and cooling cycles to permit different temperature-dependent reactions, specifically, DNA melting and enzyme-driven DNA replication. Three different steps involved in each cycle of PCR are: The denaturation of the template into single strands. Annealing of primers to each original strand for the synthesis of a new strand. Extension of the new DNA strands from the primers through the enzyme Taq DNA polymerase.

EASY

Earn 100

The technique associated with DNA amplification is . (Polymerase chain reaction / Western blotting)

50% studentsanswered this correctly

Important Questions on Biotechnology

EASY

Biology>Applied Biology>Biotechnology>Gene Amplification (PCR)

Arrange the following steps of PCR in a correct order ?

EASY

Biology>Applied Biology>Biotechnology>Gene Amplification (PCR)

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

EASY

Biology>Applied Biology>Biotechnology>Gene Amplification (PCR)

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

HARD

Biology>Applied Biology>Biotechnology>Gene Amplification (PCR)

The following are the diagrams of two important processes used in rDNA.