MEDIUM
Earn 100

There are three basic steps in creating genetically modified organisms. Arrange these steps in the correct sequence.

a. Introduction of the identified DNA into the host.
b. Maintenance of introduced DNA in the host and transfer of the DNA to its progeny.
c. Identification of DNA with desirable genes.

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Important Questions on Principles and Processes of Biotechnology

EASY
Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?
EASY
The construction of first artificial recombinant DNA molecule was by:
EASY
Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
EASY
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
EASY
A segment of DNA molecule comprises of 546 nucleotides. How many cytosine nucleotides would be present in it if the number of adenine nucleotides is 96 ?
MEDIUM
If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to
EASY
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
EASY

rDNA is produced by joining:

*None of the options were scientifically appropriate. One of them change.*

EASY
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
In gel electrophoresis, separated DNA fragments can be visualized with the help of
MEDIUM
The core techniques enabled modern biotechnology
A) Change the phenotype of host-organism by genetic engineering
B) Chemical engineering process of vaccine production by tissue culture
C) Manufacture of specific products from eukaryotic cells in bioreactors
D) Altering the chemistry of genetic material by tissue culture
MEDIUM

Match the organism with its use in biotechnology.

(a) Bacillus thuringiensis (i) Cloning vector
(b) Thermus aquaticus (ii) Construction of first rDNA molecule
(c) Agrobacterium tumefaciens (iii) DNA polymerase
(d) Salmonella typhimurium (iv) Cry proteins