Trypsin is a protease secreted in pancreatic juice, which acts in the duodenum. If you add trypsin to a suspension of milk powder in water, the enzyme digests the protein in the milk, so that the suspension becomes clear. How could you carry out an investigation into the effect of pH on the rate of activity of trypsin? (A suspension of 4g of milk powder in 100cm' of water will become clear in a few minutes if an equal volume of a 0.5% trypsin solution is added to it.)
Important Questions on Enzymes
Sketch the shape that would be in the given graph, if excess hydrogen peroxide were not available. (Celery extract contains catalase enzyme).
Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes
When free in solution
Outline an investigation you could carry out to compare the temperature at which the enzyme lactase is completely denatured within ten minutes,
When immobilised in alginate beads
Outline an experiment you could carry out to investigate how long it takes the enzyme lactase to denature at 90°C
When free in solution
Outline an experiment you could carry out to investigate how long it takes the enzyme lactase to denature at 90°C
When immobilised in alginate beads.
Outline how you would determine the optimum pH of the enzyme lactase
When free in solution
Outline how you would determine the optimum pH of the enzyme lactase
When immobilised in alginate beads.
