EASY
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What are four methods of gene transfer?

Important Questions on Biotechnology and Bioinformatics

MEDIUM
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The correct combination pair of given strategies in the area of health care developed by the rDNA technological processes.

(I) ELISA Antigen-antibody interaction
(II) RNA interference Cellular defence
(III) PCR Gene therapy
(IV) Transgenic potato Pseudomonas resistance
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Plants genes have been altered by manipulation for these uses
I) Increased efficiency of mineral usage
II) Alternative resources to industries
III) Enhanced nutritional value of food
IV) Better management practices and uses of agrochemicals
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is
HARD
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How can we make a host cell competent to receive a foreign gene or DNA?
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
The crops engineered with Bt-toxin are resistant/tolerant to:
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Biolistics method is suitable for gene transfer into ___.
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture

Match the following lists:

  List I   List II
A) Transgenic papaya I) Antigen-antibody interaction
B) Super weeds II) Ring spot virus
C) Soya bean III) Gene pollution leads to resistance to weedicides
D) ELISA IV) Herbicide tolerant

The correct match is

EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Which of the following is the first step for genetically modifying an organism?
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Which part of the tobacco plant is infected by Meloidogyne incognita?
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Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
What is the importance of E.Coli in biotechnology ?
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Name a small circular DNA molecule found in bacteria and other cells. 
MEDIUM
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What does 'competent' refer to in 'competent cells' used in transformation experiments?
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
What does 'competent' refer to in competent cells used in transformation experiments?
EASY
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
DNA can be introduced into any cell by:
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture

Transgenic plants are plants in which one or more genes from another species have been introduced into the genome, using genetic engineering. Some have been modified to resist pests. But corn is a variant of maize that expresses Cry protein from the bacterium Bacillus thuringiensis. This protein is poisonous to certain insects.

Suggest one reason mRNA was not detected in plant 3.

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HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Explain the methods of gene transfer with the help of a labelled diagram.
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Would all the bacteria emitting fluorescence have taken up the gene that it is expected was inserted into them? Explain your answer.
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
A gene construct is one or more genes together with regulatory base sequences which are inserted into a vector to produce recombinant DNA. Gene constructs often contain a marker gene, such as enhanced green fluorescent protein ( GFP). Explain why marker genes, such as enhanced GFP, are included in gene constructs.
HARD
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Why must bacterial cells be first made 'competent' in rDNA technology? How is the process carried?
 
MEDIUM
Biology>Biotechnology>Biotechnology and Bioinformatics>Biotechnology in Agriculture
Why DNA cannot pass through the cell membrane? Explain how is a bacterial cell made competent to take up recombinant DNA from the medium.