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Earn 100

What are marker genes? Give example.

Important Questions on Biotechnology - Principles and Processes

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which among the following is a selectable marker in pBR – 322 ?
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Give an account of the Blue-White Method of selection of recombinants.
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
By particles of which metal recombinant DNA is coated in particle bombardment?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Explain any two methods for introduction of recombinant DNA. into host cell.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Write the methods to introduce alien DNA into Host cells.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Write short note on the following :

Gene gun

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In biotechnology applications, a selectable marker is incorporated in a plasmid
HARD
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Describe two vector less methods of gene transfer used in rDNA technology.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Choose the wrong statement from the following:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
An antibiotic resistance gene in a vector usually helps in the selection of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is gene-gun?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is not a feature of the plasmids?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The construction of the first recombinant DNA emerged from the possibility of linking a gene encoding antibiotic resistance with a native plasmid of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In cloning vectors, antibiotic resistance genes are helpful for.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is