EASY
Earn 100

What are palindromes? Give example. 

Important Questions on Biotechnology - Principles and Processes

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In a restriction enzyme digestion experiment, a DNA fragment of 1 kilobases length is used. This fragment harbors three 5'-GAATTC-3' restriction sites. After the restriction digestion of this fragment with EcoRI, 3 DNA fragments corresponding to 500, 300 & 100 base pairs are observed in the agarose gel electrophoresis AGE. Predict the positions of the restriction site (GAATTC) in the undigested 1 kb-fragment which will result in the observed bands in AGE.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Enzyme which joins two ends of DNA strand is 
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Study the following lists:

  List-I   List-II
A. Eco RI I. Vector for transgenic plants
B. Ti plasmid II. Restriction enzyme
C. pBR 322 III. Probe
D. Reverse transcriptase IV. RNA dependent DNA synthesis
    V. Artificial plasmid

The correct match is

EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Match the following lists:

  List I   List II
A) Hind II I) pBR 322
B) Eco RI II) Replication of the plasmid
C) Hind III III) First restriction endonuclease
D) rop codes IV) E. coli Ry 13

The correct match is

MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Write down the functions of restriction enzyme and ligase enzyme. 
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Discuss the importance of cloning vectors in biotechnology.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In gel electrophoresis, separated DNA fragments can be visualized with the help of
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
MEDIUM
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the wrong statement with regard to restriction enzymes.
EASY
Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?