EASY
Earn 100

What is meant by horizontal gene transfer HGT )?

Important Questions on Consequences

MEDIUM
Which among the following is a selectable marker in pBR – 322 ?
HARD
Give an account of the Blue-White Method of selection of recombinants.
HARD
By particles of which metal recombinant DNA is coated in particle bombardment?
MEDIUM
Explain any two methods for introduction of recombinant DNA. into host cell.
EASY
109 bacteria were spread on an agar plate containing penicillin. After incubation overnight at 37oC 10 bacterial colonies were observed on the plate. That the colonies are likely to be resistant to penicillin can be tested by
EASY
Write the methods to introduce alien DNA into Host cells.
EASY

Write short note on the following :

Gene gun

EASY
Which of the following is the first step for genetically modifying an organism?
EASY
In biotechnology applications, a selectable marker is incorporated in a plasmid
HARD
Describe two vector less methods of gene transfer used in rDNA technology.
MEDIUM
Choose the wrong statement from the following:
EASY
A gene whose expression helps to identify transformed cell is known as
EASY
An antibiotic resistance gene in a vector usually helps in the selection of
MEDIUM

Assertion (A): Chromogenic substrates give blue-coloured colonies if the plasmid does not have an insert.

Reason (R): Recombinant colonies produce colour in presence of the chromogenic substrates due to the presence of β galactosidase.
The correct option among the following is:

EASY
In cloning vectors, antibiotic resistance genes are helpful for.
EASY
The technique of bombarding plant cells with high velocity microparticle's of gold or tungsten, coated with DNA, is
MEDIUM
The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
MEDIUM
The cos ends of DNA of lambda phage has ________ nucleotides.
EASY
Which of the following statement is not true for a clone?