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Earn 100

What is pBR322 plasmid?

Important Questions on Genetic Technology

HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
What are restriction endonucleases? Give the rules of their nomenclature.
HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain briefly the various process of recombinant DNA (rDNA) technology.
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
The first human hormone produced by recombinant DNA technology is:
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Diagrammatically represent recombinant DNA technology.
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain briefly the different process of recombinant DNA technology.
MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain recombinant DNA technique.
HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain briefly the various process of recombinant DNA technology.
HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Recombinant DNA technology is a complex process that involves several steps. Write down the major steps in recombinant DNA technology. 
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain the processes involved in Recombinant DNA technology
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
with reference to gel electrophoresis, what is elution?
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
What is recombinant DNA technology? Write its steps. 
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
What are transgenic bacteria? Illustrate using an example.
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Which of the following is not a characteristic of pBR322 vector?
HARD
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Explain briefly the various processes of recombinant DNA technology?
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist

Describe briefly the following

a) Origin of replication

MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
What are the steps in the process of recombinant DNA technology?
MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
How many statements are correct.
(a) Origin of replication is a sequence from where replication starts.
(b) Ori is responsible for controlling the copy number of linked DNA.
(c) For recovery of many copies of target DNA vector should have low copy number.
(d) Selectable marker helps in identifying & elimination transformants & selectively permitting the growth of non transformants.
MEDIUM
Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Insertion of foreign DNA fragment into the pBR322 using enzyme Pvu I or Pst I place make tetR nonfunctional.
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Biology>Genetics and Evolution P2>Genetic Technology>Tools for the Gene Technologist
Describe various steps of recombinant DNA technique of genetic engineering.