EASY
Earn 100

What is pBR322 plasmid?

Important Questions on Genetic Technology

HARD
What are restriction endonucleases? Give the rules of their nomenclature.
HARD
Explain briefly the various process of recombinant DNA (rDNA) technology.
EASY
The first human hormone produced by recombinant DNA technology is:
HARD
Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
EASY
Diagrammatically represent recombinant DNA technology.
HARD
Explain briefly the different process of recombinant DNA technology.
HARD
Explain briefly the various process of recombinant DNA technology.
HARD
Recombinant DNA technology is a complex process that involves several steps. Write down the major steps in recombinant DNA technology. 
HARD
Explain the processes involved in Recombinant DNA technology
EASY
with reference to gel electrophoresis, what is elution?
HARD
What is recombinant DNA technology? Write its steps. 
HARD
What are transgenic bacteria? Illustrate using an example.
EASY
Which of the following is not a characteristic of pBR322 vector?
HARD
Explain briefly the various processes of recombinant DNA technology?
HARD

Describe briefly the following

a) Origin of replication

MEDIUM
What are the steps in the process of recombinant DNA technology?
MEDIUM
How many statements are correct.
(a) Origin of replication is a sequence from where replication starts.
(b) Ori is responsible for controlling the copy number of linked DNA.
(c) For recovery of many copies of target DNA vector should have low copy number.
(d) Selectable marker helps in identifying & elimination transformants & selectively permitting the growth of non transformants.
MEDIUM
Insertion of foreign DNA fragment into the pBR322 using enzyme Pvu I or Pst I place make tetR nonfunctional.
HARD
Describe various steps of recombinant DNA technique of genetic engineering.