EASY
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What is the definition of gene cloning?

Important Questions on Breeding and Biotechnology

EASY
What is the importance of E.Coli in biotechnology ?
HARD
How can we make a host cell competent to receive a foreign gene or DNA?
HARD
Would all the bacteria emitting fluorescence have taken up the gene that it is expected was inserted into them? Explain your answer.
MEDIUM
Why DNA cannot pass through the cell membrane? Explain how is a bacterial cell made competent to take up recombinant DNA from the medium.
EASY
Choose the correct pair of cell wall digesting enzymes used in organisms to separate DNA.
EASY
Name a small circular DNA molecule found in bacteria and other cells. 
HARD
Explain the methods of gene transfer with the help of a labelled diagram.
HARD
A gene construct is one or more genes together with regulatory base sequences which are inserted into a vector to produce recombinant DNA. Gene constructs often contain a marker gene, such as enhanced green fluorescent protein ( GFP). Explain why marker genes, such as enhanced GFP, are included in gene constructs.
HARD
Why must bacterial cells be first made 'competent' in rDNA technology? How is the process carried?
 
MEDIUM
λ Bacteriophage is a better vector than plasmids.
MEDIUM
Describe the role of CaCl2 in the preparation of competent cells.
MEDIUM
λ Bacteriophage is more important as vector than M13 because:
MEDIUM
What does 'competent' refer to in 'competent cells' used in transformation experiments?
MEDIUM
What does 'competent' refer to in competent cells used in transformation experiments?
HARD

Transgenic plants are plants in which one or more genes from another species have been introduced into the genome, using genetic engineering. Some have been modified to resist pests. But corn is a variant of maize that expresses Cry protein from the bacterium Bacillus thuringiensis. This protein is poisonous to certain insects.

Suggest one reason mRNA was not detected in plant 3.

Question Image

EASY
DNA can be introduced into any cell by:
EASY
With a neat labelled diagram explain the techniques involved in gene cloning.
EASY
Which of the following match is correct for the enzyme used to extract DNA?
HARD
How is a bacterial cell made 'competent' to take up DNA?
MEDIUM

Represent the union of two sets by Venn diagram for each of the following.

X={x | x is a prime number between 80 and 100}

Y={y | y is an odd number between 90 and 100}