EASY
Earn 100
What is the nomenclature of restriction enzymes?
Important Questions on Biotechnology - Principles and Processes
HARD
What are restriction endonucleases? Give the rules of their nomenclature.
HARD
Give an account of the Blue-White Method of selection of recombinants.
HARD
Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
MEDIUM
Explain what are the desired characteristics of an ideal cloning vector used in rDNA technology.
HARD
Describe two vector less methods of gene transfer used in rDNA technology.
MEDIUM
Which of the following tools are required for preparation of a recombinant DNA molecule?
EASY
In the above diagram of bacteriophage "X" represent _____ (Cap / Head / Tip)
EASY
Which of the following statement is correct regarding EcoRI restriction endonuclease enzyme?
MEDIUM
Which of the following is true regarding the phage lambda, a virus which infects bacteria?
EASY
In another method, suitable for plants, cells are bombarded with high velocity micro-particles of gold or tungsten coated with DNA in a method known as _____or gene gun.
EASY
DNA _____ enzymes seal the nicks between adjacent nucleotides in double stranded DNA molecules.
EASY
The extrachromosomal circular DNA of bacteria used as a vector in genetic engineering is called _____.
MEDIUM
The correct statement related to the endonucleases is/are
HARD
The recombinant DNA molecule is directly injected into nucleus of an animal cell in _____(microinjection/transplantation) method.
MEDIUM
Which of the following tools are required for preparation of a recombinant DNA molecule?
EASY
The linking of antibiotic resistance gene with the plasmid vector became possible with the _____ DNA ligase, which acts on cut DNA molecules and joins their ends.
EASY
Transfer of desired DNA fragments into the host cell is done by _____. (Vector/Liposomes)
MEDIUM
Bacteriophages and _____ can replicate within the bacterial cells independent of the chromosomal DNA control.
MEDIUM
Fill in the blank with the correct answer from the bracket.
The recognition site Pvu I in pBR322 is located within the _____ gene. (tetR, ampR, EcoRI)
EASY
The most important feature in a plasmid to be used as a vector is:
