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Which enzyme is used in genetic engineering that catalyses the formation of a phosphodiester bond, whether it is ligase or helicase?
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Important Questions on Consequences
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Write short note on the following:
Tools of genetic engineering
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Match the following lists.
| List I | List II |
| A) Remove nucleotides from the end of the DNA | I) Polymerases |
| B) Restriction enzyme | II) Endonuclease |
| C) Enzyme which cuts at specific position of DNA | III) Ligase |
| D) Stick the complementary cut ends of DNA with H2 bond | IV) Nucleases |
| V) Exonucleases |
The correct match is:
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(A) In gel electrophoresis, DNA fragments resolve according to their size.
(B) It is not necessary to digest the bacterial cells with lysozyme to isolate DNA
(C) In PCR, DNA polymerase extends the primers using the nucleotides.
(D) During the isolation of DNA, ribonuclease is added along with protease
(E) Agarose gel electrophoresis is employed to check the progression of restriction enzyme digestion.
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Give one significant contribution of the following scientist:
H. Boyer
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Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
