MEDIUM
Earn 100

Which of the following are the steps of genetic engineering?
(a)Isolation of DNA
(b)Fragmentation of DNA by restriction endonuclease.
(c)Isolation of the desired DNA fragments.
(d)All of the above.

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Important Questions on Principles and Processes of Biotechnology
EASY
Genetic Engineering is used in

HARD
List four applications of genetically modified plants.

EASY
Explain Agrobacterium mediated gene transfer.

EASY
Which ONE of the following is NOT essential for Polymerase Chain Reaction (PCR)?

EASY
The construction of first artificial recombinant DNA molecule was by:

EASY
Which of the following is not required for any of the techniques of DNA fingerprinting available at present?

MEDIUM
What is genetic engineering? How is it beneficial?

EASY
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:

EASY
A segment of DNA molecule comprises of 546 nucleotides. How many cytosine nucleotides would be present in it if the number of adenine nucleotides is 96 ?

EASY
Write short note on the following:
Gene cloning

MEDIUM
If the initial number of templets DNA molecules in a PCR reaction is 1000, the number of product DNA molecules at the end of 20 cycles will be closest to

EASY
H. Boyer S. Cohen are famous for their research work on

EASY
The DNA fragments separated on an agarose gel can be visualised after staining with:

EASY
rDNA is produced by joining:
*None of the options were scientifically appropriate. One of them change.*

EASY
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?

EASY
In gel electrophoresis, separated DNA fragments can be visualized with the help of

HARD
What is DNA library?

MEDIUM
The core techniques enabled modern biotechnology
Change the phenotype of host-organism by genetic engineering
Chemical engineering process of vaccine production by tissue culture
Manufacture of specific products from eukaryotic cells in bioreactors
Altering the chemistry of genetic material by tissue culture

MEDIUM
Match the organism with its use in biotechnology.
(a) | Bacillus thuringiensis | (i) | Cloning vector |
(b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
(c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
(d) | Salmonella typhimurium | (iv) | Cry proteins |

