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Which of the following enzymes is used to remove the phosphate group from the 5' end of DNA molecule, leaving a free 5' hydroxyl group, so that it cannot be ligated to another DNA fragment?

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Important Questions on Biotechnology: Principles and Processes

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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 5
The enzyme used to combine two fragments of DNA is:
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 6
Who discovered the reverse transcriptase an enzyme used to synthesize complementary DNA (cDNA) by using mRNA as a template?
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 7
Which of the following is an incorrect statement?
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 8
Rejoining of vector molecules after restriction enzyme digestion can be avoided by using:
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 9
The specific DNA sequence where EcoRI cuts is:
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 10
The role of DNA ligase in the construction of recombinant DNA molecule is:
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 11
Which of the following is correctly matched?
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Chapterwise/Topicwise Daily Practice Problems (DPP) Unit IX: Biotechnology Unit X: Ecology NEET>Chapter 1 - Biotechnology: Principles and Processes>Exercise 1>Q 12
Which of the following is not a vector-less method of gene transfer?