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Which of the following enzymes is used to remove the phosphate group from the 5' end of DNA molecule, leaving a free 5' hydroxyl group, so that it cannot be ligated to another DNA fragment?
(a)DNA ligase
(b)DNA polymerase
(c)Alkaline phosphatase
(d)Restriction endonuclease

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Important Questions on Biotechnology: Principles and Processes
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The enzyme used to combine two fragments of DNA is:

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Who discovered the reverse transcriptase an enzyme used to synthesize complementary DNA (cDNA) by using mRNA as a template?

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Which of the following is an incorrect statement?

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Rejoining of vector molecules after restriction enzyme digestion can be avoided by using:

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The specific DNA sequence where EcoRI cuts is:

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The role of DNA ligase in the construction of recombinant DNA molecule is:

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Which of the following is correctly matched?

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Which of the following is not a vector-less method of gene transfer?
