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Which of the following is not used as a vector in genetic engineering?

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Important Questions on Biotechnology - Principles and Processes

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In gel electrophoresis, separated DNA fragments can be visualized with the help of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Restriction endonuclease recognises palindromic nucleotide sequences, because they have characteristic
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Ti plasmid is obtained from ___.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R)： The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to UV - radiation.

Which of the following is true?

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Identify the wrong statement with regard to restriction enzymes.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In a restriction enzyme digestion experiment, a DNA fragment of 1 kilobases length is used. This fragment harbors three 5'-GAATTC-3' restriction sites. After the restriction digestion of this fragment with EcoRI, 3 DNA fragments corresponding to 500, 300 & 100 base pairs are observed in the agarose gel electrophoresis AGE. Predict the positions of the restriction site (GAATTC) in the undigested 1 kb-fragment which will result in the observed bands in AGE.
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Study the following lists:

  List-I   List-II
A. Eco RI I. Vector for transgenic plants
B. Ti plasmid II. Restriction enzyme
C. pBR 322 III. Probe
D. Reverse transcriptase IV. RNA dependent DNA synthesis
    V. Artificial plasmid

The correct match is

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector pBR322 is
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The introduction of T-DNA into plants involves :