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Earn 100

Which of the following is not used to transfer the recombinant DNA into the host?

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Important Questions on Biotechnology: Principles and Processes

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A device in which large volume of living cells are cultured in order to get a specific product is called
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Which of the following statements are correct with respect to a bioreactor?
(i) It can process large volumes of culture.
(ii) It provides optimum temperature and pH.
(iii) It is a completely automated tool.
(iv) It is a compact thermal cycler.
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Identify the figures (A) and (B) and select the correct option:
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Stirred-tank bioreactors have advantages over shake flasks because they
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After completion of the biosynthetic stage in the bioreactors, the product undergoes separation and purification processes, collectively termed as
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Study the following statements regarding recombinant DNA technology and select the incorrect ones.
(i) Taq polymerase extends the primers using the nucleotides provided in the reaction.
(ii) Antibiotic resistance genes are considered as desirable genes in recombinant DNA technology.
(iii) DNA fragments are separated according to their charge only, in agarose gel electrophoresis.
(iv) Transformation is a procedure through which a piece of DNA is integrated into the genome of a host bacterium.
(v) To produce higher yields of a desired protein, host cells can be multiplied in a continuous culture.
(vi) Downstream processing is one of the steps of polymerase chain reaction.
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Read the following statements and select the incorrect ones.
(i) When the transformed cells on agar plates containing ampicillin are spread, both transformed and untransformed cells will grow.
(ii) Restriction enzymes are used in isolation and separation of DNA from other macromolecules.
(iii) Downstream processing is one of the steps of rDNA technology.
(iv) Disarmed pathogen vectors are also used in the transfer of rDNA into the host.
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Match the scientists in Column I with their related\discoveries in Column II and select the correct option from the given codes.
  Column I   Column II
(a) Kary Mullis (i) Father of genetic engineering
(b) Paul Berg (ii) Nobel Prize for the discovery of restriction endonucleases
(c) Stanley Cohen and Herbert Boyer (iii) Developed polymerase chain reaction
(d) Arber, Smith and Nathan (iv) Isolated an antibiotic resistant gene from a plasmid of the bacterium Salmonella typhimurium