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Earn 100
Which of the following is required for micro-injection method of gene transfer?
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Important Questions on Biotechnology: Principles and Processes
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In biolistic method of gene transfer, the microparticles coated with foreign DNA are bombarded into target cells at a very high velocity. These microparticles are made up of
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The different steps of recombinant DNA technology are given below randomly.
(i) Isolation of the DNA fragments or genes to be cloned
(ii) Introduction of the recombinant DNA into a suitable cell (usually E. coli) called host (transformation)
(iii) Multiplication/expression of the introduced gene in the host
(iv) Selection of the transformed host cells, and identification of the clone containing the desired gene/DNA fragment
(v) Insertion of the isolated gene in a suitable plasmid vector
Which of the following represents the correct sequence of steps?
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Observe the steps to transfer a desirable gene of interest into a plant.
Identify the missing steps (, and ) with regard to following statements and select the correct match.
(i) Joining of desirable gene to a suitable cloning vector using ligases to create a recombinant DNA molecule.
(ii) Selection of transformed cells.
(iii) Transferring the recombinant molecules to the target cells.
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Fill up the blanks and select the correct option.
(i) EcoRI cuts the DNA between bases ____ only when the sequence ____ is present in the DNA duplex.
(ii) Disruption of the cell membranes can be achieved by treating the bacterial cells, plant cells and fungal cells with enzymes respectively _____, _____ and_____.
(iii) Since DNA has a _____ charge, it moves towards the _____ of the electrophoretic chamber.
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In the isolation of DNA, removal of protein and RNA is carried out by enzymes _____ and _____, respectively.
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During isolation of genetic material, the chemical used to precipitate out the purified DNA is
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Precipitates of purified DNA after the addition of chilled ethanol are seen as a collection of fine threads in suspension. This process is referred as
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Match Column I with Column II and select the correct answer from the given codes.
| Column I | Column II | ||
|---|---|---|---|
| Recombinant DNA technology | Chilled ethanol | ||
| Precipitation of DNA | DNA staining | ||
| Transposons | Jumping genes | ||
| Ethidium bromide | Genetic engineering |
