EASY
Earn 100
Which of the following statement is correct regarding EcoRI restriction endonuclease enzyme?
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Important Questions on Biotechnology - Principles and Processes
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The ratio of genes for protein involved in plasmid replication rop codes for proteins, Ori site, restriction sites, antibiotic resistance genes present in E. coli cloning vector is
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Which vector can clone only a small fragment of DNA?
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plasmid is obtained from ___.
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What is endonuclease? What do you mean by restriction site?
MEDIUM
Explain what are the desired characteristics of an ideal cloning vector used in rDNA technology.
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What do you mean by cDNA. What is transfection?
MEDIUM
Match the organism with its use in biotechnology.
| (a) | Bacillus thuringiensis | (i) | Cloning vector |
| (b) | Thermus aquaticus | (ii) | Construction of first rDNA molecule |
| (c) | Agrobacterium tumefaciens | (iii) | DNA polymerase |
| (d) | Salmonella typhimurium | (iv) | Cry proteins |
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Which of the following is commonly used as a vector for introducing a DNA fragment in human lymphocytes?
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Give an account of the Blue-White Method of selection of recombinants.
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Commonly used vectors for human genome sequencing are:
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Which one of the following is an example of restriction endonuclease enzyme?
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Explain the mechanism of the action of restriction endonucleases that makes them suitable for genetic engineering.
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The cloning vector PBR322 used in genetic engineering is a/an
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In biotechnology, which enzymes are called ‘molecular scissors’ and ‘molecular glue’?
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Describe the essential steps in genetic engineering.
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The cutting of DNA at a specific location became possible with the discovery of:
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Which of the following is a restriction endonuclease?
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DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
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What are restriction endonucleases? Give the rules of their nomenclature.
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Describe two vector less methods of gene transfer used in rDNA technology.
