Which of the following statements are correct?
PCR is now routinely used to detect HIV in suspected AIDS patients.
A single-stranded DNA or RNA, tagged with a radioactive molecule (probe) is allowed to hybridize to its complementary DNA in a clone of cells followed by detection using autoradiography.
ELISA is based on the principle of antigen-antibody interaction.
Today transgenic models exist for many human diseases such as cancer, cystic fibrosis, rheumatoid arthritis, and Alzheimer's.
Important Questions on Biotechnology and its Applications
Identify the correct sequence of steps that are followed in gene therapy.
A Altered virus is mixed with cells from patient
B. Therapeutic human gene is inserted into the virus (vector)
C. Cells are collected from the patients
D. Cells from the patient become genetically altered
E. Reintroduction of genetically altered cells into patient
F. Suitable virus (vector) is selected and altered
G The genetically altered cells produce the desired proteins or hormones
Match the following columns and select the correct option.
| Column-I | Column-II | ||
| (a) | Bt cotton | (i) | Gene therapy |
| (b) | Adenosine deaminase deficiency | (ii) | Cellular defence |
| (c) | RNAi | (iii) | Detection of HIV infection |
| (d) | PCR | (iv) | Bacillus thuringiensis |
Match the following
| List - I | List - II | ||
| (a) | First clinical gene therapy | (i) | DNA Amplification |
| (b) | Transfection | (ii) | Introduction of genetically modified DNA into a eukaryotic cell |
| (c) | Synthetic oligodeoxynucleotides | (iii) | Adenosine deaminase |
| (d) | PCR | (iv) | Silencing genes |
The correct answer is
With regard to insulin choose correct options.
(a) C-peptide is not present in mature insulin.
(b) The insulin produced by rDNA technology has C-peptide.
(c) The pro-insulin has C-peptide.
(d) A-peptide and B-peptide of insulin are interconnected by disulphide bridges.
