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Which of the following statements is false regarding SDS-polyacrylamide gel electrophoresis?

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Important Questions on Biotechnology - Principles and Processes

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Which of the following is not required for any of the techniques of DNA fingerprinting available at present?
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Which one among the following primer pairs would amplify the fragment of DNA given below?

5'-CTAGTCGTCGAT-N300-GACTGAGCTGAGCTG-3'

3'·GATCAGCAGCTA-(N)300-CTGACTCGACTCGAC-5'

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dsRNA is used to develop pest resistant tobacco plant by a technique called
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Select the correct steps involved in amplification of gene
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Silencing of a specific mRNA translation could be achieved through
HARD
Plasmid pBR322 has PstI restriction enzyme site within gene ampR that confers ampicillin resistance. If this enzyme is used for inserting a gene for β-galactoside production and the recombinant plasmid is inserted in an E. coli strain
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Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R): The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to UV - radiation.

Which of the following is true?

EASY
Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
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The DNA fragments separated on an agarose gel can be visualised after staining with:
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Identify the labels M and N in the following Agarose gel electrophoresis representation.

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DNA fragments of 100 base pairs (bp), 300 bp and 500 bp were separated by agarose gel electrophoresis. Pick the correct arrangement of the fragments separated on the gel in the increasing order of their migration from the wells.
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r-DNA inserted within a coding sequence results in the process ________
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Identify the labeled parts 1, 2, 3, 4 in the given figure?

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The core techniques enabled modern biotechnology
A) Change the phenotype of host-organism by genetic engineering
B) Chemical engineering process of vaccine production by tissue culture
C) Manufacture of specific products from eukaryotic cells in bioreactors
D) Altering the chemistry of genetic material by tissue culture
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In gel electrophoresis, separated DNA fragments can be visualized with the help of
EASY
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?