
Which one of the following vectors is used to replace the defective gene in gene therapy?

Important Questions on Principles and Process of Biotechnology


Change the phenotype of host-organism by genetic engineering
Chemical engineering process of vaccine production by tissue culture
Manufacture of specific products from eukaryotic cells in bioreactors
Altering the chemistry of genetic material by tissue culture



Dissolution of biological membranes
Enzymatic digestion of cell wall
Precipitation of DNA
Removal of DNA by spooling

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
A. Insertion of recombinant DNA into the host cell.
B. Cutting of DNA at specific location by restriction enzyme.
C. Isolation of desired DNA fragment.
D. Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:






Biotechnologists refer to which of the following bacteria as a natural genetic engineer of plants.

Agrobacterium mediated genetic transformation described as natured genetic engineering is used to resist plants from:


Represent the union of two sets by Venn diagram for each of the following.
is a prime number between and
is an odd number between and


Read the following statements and choose the correct option:
Statement I: Agrobacterium tumefaciens, a pathogen of several monocot plants, is able to deliver a piece of DNA known as “T-DNA” to transform normal plant cells into tumors.
Statement II: The tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens has now been modified into a cloning vector, which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.



