EASY
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Which one of the following vectors is used to replace the defective gene in gene therapy?

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Important Questions on Principles and Process of Biotechnology

EASY
The construction of first artificial recombinant DNA molecule was by:
MEDIUM
The core techniques enabled modern biotechnology
A) Change the phenotype of host-organism by genetic engineering
B) Chemical engineering process of vaccine production by tissue culture
C) Manufacture of specific products from eukaryotic cells in bioreactors
D) Altering the chemistry of genetic material by tissue culture
MEDIUM
Which enzymes are responsible for breaking the wall of bacterial cell, plant cell, and fungus cell respectively?
MEDIUM
"Agrobacterium is a natural genetic engineer of plants." Analyse it with two points.
EASY
Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
EASY

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

A. Insertion of recombinant DNA into the host cell.

B. Cutting of DNA at specific location by restriction enzyme.

C. Isolation of desired DNA fragment.

D. Amplification of gene of interest using PCR.

Choose the correct answer from the options given below: 

EASY
Fragments of DNA can be separated by a technique known as:
MEDIUM
It is used for the entry of the desired gene in animal cells.
EASY
DNA's precipitation out of a mixture of biomolecules can be achieved by treatment with:
EASY
Mark the type of culturing method which is commonly used to produce a larger biomass leading to higher yields of desired proteins?
HARD

Biotechnologists refer to which of the following bacteria as a natural genetic engineer of plants.

EASY

Agrobacterium mediated genetic transformation described as natured genetic engineering is used to resist plants from:

MEDIUM

Represent the union of two sets by Venn diagram for each of the following.

X={x | x is a prime number between 80 and 100}

Y={y | y is an odd number between 90 and 100}

MEDIUM
The most thoroughly studied known bacteria–plant interaction is:
MEDIUM

Read the following statements and choose the correct option:

Statement I: Agrobacterium tumefaciens, a pathogen of several monocot plants, is able to deliver a piece of DNA known as “T-DNA” to transform normal plant cells into tumors.

Statement II: The tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens has now been modified into a cloning vector, which is no more pathogenic to the plants but is still able to use the mechanisms to deliver genes of our interest into a variety of plants.

EASY
Which of the following is an incorrect pair for the organism and its cell wall degrading enzyme?
EASY
While isolating DNA from bacteria, which of the following enzymes is not required?