Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 13

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Why would lac $z$ gene be fused to coding sequence of chain $A$ and $B$ in plasmids of E.coli?

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Important Questions on Biotechnology - Principles and Processes

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 1

Melting of DNA at

94 ° C

is due to breakdown of

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 3

Plasmids are suitable vectors for gene cloning because they

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 4

EcoRI

recognizes the palindrome

5^{'}

GAATTC

3^{'}

. How many fragments are obtained upon digesting

pBR 322

with

Sal I

?

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 6

Read statements A and B and select the correct option given below.

Statement-A : DNA is a hydrophilic molecule, thus it cannot pass through cell membrane.
Statement-B : Electroporation creates transient microscopic pores in cell membrane making entry of DNA molecules into the cell easier.

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 7

Tumor inducing plasmid delivers a piece of DNA known as 'T-DNA' to transform

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 8

The intercalating agent that stacks between base pairs of DNA and helps in visualizing them under UV light is

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 9

Fragments of DNA formed after treatment with endonucleases are separated by the technique of

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Gamma Question Bank for Medical Biology>Chapter 33 - Biotechnology - Principles and Processes>Assignment>Q 11

Absence of

β

-galactosidase activity in recombinant

pUC 18

is