Name: Kidney as the Body Filter
Uploaded: 2019-07-19
Duration: 4 min 4 s
Description: The kidney is the waste manager of our body. Let us look inside the kidney and understand how this wondrous filtration system works. Watch the video now.

Question

Write an experiment to prove that chlorophyll is necessary for photosynthesis.

Accepted Answer

Aim: To prove that chlorophyll pigment is necessary for photosynthesis in the plants.
Requirements :
A well-watered variegated leaf plant, beaker, water, Bunsen burner, mentholated spirit or rectified spirit or ethanol, iodine solution, a white porcelain tile.

Theory:
Photosynthesis is a physiological process of green plants which helps to prepare their own food or carbohydrates. To prove chlorophyll is necessary for photosynthesis, an important step has to be carried out is known as De-starching. De-starching is a step in which the starch present in the leaves of the plant will be removed by keeping the variegated leaves plant in a dark room or closed cupboard for about 2-3 days.

Procedure:
1. A variegated leaf plant is taken.
2. The place where green patches are present in the leaf, chlorophyll pigment is present while at the other colour patches present in the leaf contain other pigments but not chlorophyll.
3. Before starting the experiment to prove that chlorophyll is necessary for photosynthesis, an important step has to be carried out is known as De-starching.

4. This step is done to ensure that previously synthesized starch in the leaves get consumed up because photosynthesis doesn't occur at that time as the plant is kept in the dark place.
5. After De-starching step, the variegated leaf plant is kept in bright sunlight for about 6 hours.
6. After this, one of the variegated leaves is plucked out from the experimental plant.
7. Then Iodine test is conducted for this experimental leaf to find out whether the leaf prepared starch through photosynthesis or not.

Fig: showing starch test (blue-black colour) before photosynthesis

Observation: The green coloured leave will turn blue-black in colour showing that starch is being formed or photosynthesis is carried out. The yellow leaf doesn't turn blue-black in colour showing that starch is not formed in or photosynthesis is not carried out.
Result:
The chlorophyll pigment is necessary for photosynthesis in the plants.

Write an experiment to prove that chlorophyll is necessary for photosynthesis.

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