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(Agarose/SDS) gel electrophoresis is commonly used to separate DNA.

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Important Questions on Biotechnology - Principles and Processes

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is a restriction endonuclease?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The core techniques enabled modern biotechnology
A) Change the phenotype of host-organism by genetic engineering
B) Chemical engineering process of vaccine production by tissue culture
C) Manufacture of specific products from eukaryotic cells in bioreactors
D) Altering the chemistry of genetic material by tissue culture
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

VNTR is

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A gene whose expression helps to identify transformed cell is known as
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
In gel electrophoresis, separated DNA fragments can be visualized with the help of
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
A foreign DNA and plasmid cut by the same restriction endonuclease can be joined to form a recombinant plasmid using
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Which one among the following primer pairs would amplify the fragment of DNA given below?

5'-CTAGTCGTCGAT-N300-GACTGAGCTGAGCTG-3'

3'·GATCAGCAGCTA-(N)300-CTGACTCGACTCGAC-5'

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which vector can clone only a small fragment of DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Select the correct steps involved in amplification of gene
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Assertion (A): The fragments of DNA generated by the action of restriction endonucleases are separated by polyacrylamide gel electrophoresis.

Reason (R)： The separated DNA fragments are visualised as bright orange coloured bands after staining with Ethidium Bromide followed by exposure to UV - radiation.

Which of the following is true?

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which one of the following represents a palindromic sequence in DNA?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
What is the following is correct for the movement of DNA fragments on agarose gel during gel electrophoresis?
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology

Following statements describe the characteristics of restriction endonucleases. Identify the incorrect statement.

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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The cutting of DNA at a specific location became possible with the discovery of:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Commonly used vectors for human genome sequencing are:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Write down the functions of restriction enzyme and ligase enzyme. 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Enzyme which joins two ends of DNA strand is 
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Sequence of strategies involved for the isolation of gene of interest from a plant cell during the process of rDNA technology
A) Dissolution of biological membranes
B) Enzymatic digestion of cell wall
C) Precipitation of DNA
D) Removal of DNA by spooling
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
The DNA fragments separated on an agarose gel can be visualised after staining with:
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Biology>Biotechnology>Biotechnology - Principles and Processes>Tools of Recombinant DNA Technology
Which of the following is not required for any of the techniques of DNA fingerprinting available at present?