Transcription

IMPORTANT

Transcription: Overview

This Topic covers sub-topics such as RNA Polymerase, Exons, Introns, Transcription Unit, Coding Strand, Template Strand, Transcription of DNA, Initiation of Transcription, Termination of Transcription, hnRNA and, Elongation of Transcription

Important Questions on Transcription

EASY
IMPORTANT

TATA box of eukaryotic promotor lies

HARD
IMPORTANT

Choose the correct statements from the following.

A) Coding strand in DNA will be coding the m-RNA.

B) If two RNA molecules are produced simultaneously, they will form a double stranded RNA.

C) In prokaryotes, the structural gene is polycistronic.

D) The expressed gene sequences are defined as exons.

HARD
IMPORTANT

Identify the correct statement:

EASY
IMPORTANT

Which ONE of the following polymerases transcribes 5S rRNA?

MEDIUM
IMPORTANT

Which type of RNA polymerase enzyme transcribes precursor mRNA?

MEDIUM
IMPORTANT

Which type of RNA polymerase enzyme transcribes precursor mRNA? 

EASY
IMPORTANT

Find out the series of genes in the transcription unit of the following statements.

I. Located towards 3 '-end of the structural gene.

II.  Flanked by the promoter and the terminator.

III. Located towards 3'-end of the coding strand.

MEDIUM
IMPORTANT

Match the following

List-I List - II List -III
 A)  tailing  i) 28 s,18 s,5.8 s I) Transported out of nucleus
 B)  r-RNA  ii) Addition of methyl
guanosine triphosphate
II) 3 'end
C) Caping iii) m-RNA III) Transcribes by RNA polymerase
D) Processed
hn RNA
iv) Addition of Adenylate
Residue
IV) 5 ' end of hn RNA

The correct answer is

EASY
IMPORTANT

Assertion (A) : During transcription both the strands of DNA are copied

Reason (R):   If both strands act as template DNA would code for RNA molecule with different sequences

MEDIUM
IMPORTANT

Identify the correct statement:

MEDIUM
IMPORTANT

Assertion (A): The structural gene in a transcription unit can be said to be monocistronic or polycistronic.

Reason (R): In eukaryotes, the monocistronic structural gene has interrupted coding sequence - but the genes in eukaryotes split. The coding sequence or expressed sequences are defined as exons. Exons are said to be those sequence that appears in mature or processed RNA.
The correct answer is

MEDIUM
IMPORTANT

What is the corresponding sequence of mRNA when a polynucleotide template DNA strand is 3'-TAC GGT AAG CGG ATC-5'?

HARD
IMPORTANT

Read the following statements and choose the correct option.
(i) Tailing: It is addition of adenylate residues at 5' -end in a template independent manner.
(ii) Promoter: It is the site recognised by the RNA polymerase in association with ρ factor.
(iii) Division of labour: RNA polymerase I and RNA polymerase III are responsible for 18S rRNA and tRNA transcription respectively.
(iv) Capping: addition of methyl guanosine triphosphate at the 5'-end of hnRNA.

EASY
IMPORTANT

If the template strand of DNA is 3'ATGCATGCATGCATGC 5', identify the sequence of RNA transcribed.

HARD
IMPORTANT

Choose the correct statements.
A) tRNA looks like an inverted 'L'.
B) Single DNA dependent RNA polymerase catalyses the transcription of all types of RNA in eukaryotes.
C) The structural gene in a transcription unit is polycistronic in prokaryotes.
D) DNA polymerase catalyses the polymerization of DNA in 5'3' direction where 3'5' strand acts as a template.

EASY
IMPORTANT

Find out the series of genes in the transcription unit of the following statements

(I) Located towards 3 ' end of the structural gene

(II) Flanked by promoter and terminator

(III) Located towards 3 ' end of the coding strand

HARD
IMPORTANT

On which strand of DNA, mRNA is constructed?

EASY
IMPORTANT

Which one of the following does not follow the central dogma of molecular biology?

MEDIUM
IMPORTANT

The following statements are with respect to transcription in eukaryotes. Choose the correct ones.
(i) The strand of dsDNA which takes part in transcription process is called as coding strand.
(ii) The enzyme RNA polymerase can catalyse polymerisation only in one direction i.e., 5'3' .
(iii) An unusual nucleotide methyl guanosine triphosphate is added to the 5' end of hnRNA during capping.
(iv) During tailing process, adenylate residues 200-300 are added at 3' end in a template independent manner.

EASY
IMPORTANT

In which of the following direction/s will the the enzyme DNA dependent RNA polymerase catalyse polymerisation reaction?