HARD
AS and A Level
IMPORTANT
Earn 100
Explain the advantage of using Crispr/Cas9 over using restriction enzymes.
Important Questions on Genetic Technology
HARD
AS and A Level
IMPORTANT
Describe how Crispr/Cas9 can be used to correct a genetic fault.
HARD
AS and A Level
IMPORTANT
Explain the difference between a primer and a probe.
HARD
AS and A Level
IMPORTANT
Suggest why the primers used in PCR do not anneal together.
MEDIUM
AS and A Level
IMPORTANT
State the four types of free nucleotides (dNTPs) that are added to reaction vessels at the start of the PCR process.
MEDIUM
AS and A Level
IMPORTANT
Explain why only certain DNA polymerases, such as Taq polymerase, are used in PCR.
HARD
AS and A Level
IMPORTANT
State what happens to DNA during one cycle in PCR.
HARD
AS and A Level
IMPORTANT
How many molecules of DNA are produced from one double-stranded starting molecule, after eight cycles of PCR?
HARD
AS and A Level
IMPORTANT
Explain why it is not possible to use PCR to increase the number of RNA molecules in the same way as it is used to increase the number of DNA molecules.
