Genetic engineering is
Important Questions on Principles and Processes of Biotechnology
Consider the following statements:
I. Recombinant DNA technology is popularly known as genetic engineering is a stream of biotechnology which deals with the manipulation of genetic materials by man in vitro.
II. pBR322 is the first artificial cloning vector developed in 1977 by Boliver and Rodriguez from E.coli plasmid.
III. Restriction enzymes belong to a class of enzymes called nucleases.
Choose the correct option regarding above statements.
The process of recombinant DNA technology has the following steps:
Amplification of the gene.
Insertion of recombinant DNA into the host cells.
Cutting of DNA at a specific location using a restriction enzyme.
Isolation of genetic material (DNA).
Pick out the correct sequence of steps for recombinant DNA technology.
Match the following:
| Column A | Column B |
| 1 Exonuclease | a. add or remove phosphate |
| 2 Endonuclease | b. binding the DNA fragments |
| 3 Alkaline phosphatase | c. cut the DNA at terminus |
| 4 Ligase | d. cut the DNA at middle |
Assertion: Agrobacterium tumefaciens is popular in genetic engineering because this bacterium is associated with the root nodules of all cereals and pulse crops.
Reason: A gene incorporated in the bacterial chromosomal genome gets automatically transferred to the cross with which bacterium is associated.
