Homozygous pure lines in cattle can be obtained by

Arrange the steps to be followed in breeding techniques

(A) Creating the plants as pure lines

(B) Progeny of the hybrids to be tested for the homozygosity for generations

(C) Evaluation by growing in research field, farmer field and then for three growing seasons in several locations

(D) Evaluation with best available local crop cultivars

(E) Pollen grain of desirable male plant to be collected and placed on the stigma of the selected female plant

(F) Effective exploitation of natural genes available in the population