Match the following techniques or instruments with their usage: (a) Bioreactor (i) Separation of DNA fragments (b) Electrophoresis (ii) Production of large quantities of products (c) PCR (iii) Detection of pathogen, based on antigen-antibody reaction (d) ELISA (iv) Amplification of nucleic acids Select the correct option from following:

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iv ) , ( d ) - ( iii )

( a ) - ( iii ) , ( b ) - ( ii ) , ( c ) - ( iv ) , ( d ) - ( i )

( a ) - ( iv ) , ( b ) - ( iii ) , ( c ) - ( ii ) , ( d ) - ( i )

( a ) - ( ii ) , ( b ) - ( i ) , ( c ) - ( iii ) , ( d ) - ( iv )

Which of the following is not a desirable feature of a cloning vector?

Presence of two or more recognition sites

Presence of single restriction enzyme site

Presence of origin of replication

Given below are two statements: Statement I : Restriction endonucleases recognise specific sequence to cut DNA known as palindromic nucleotide sequence. Statement II : Restriction endonucleases cut the DNA strand a little away from the centre of the palindromic site. In the light of the above statements, choose the most appropriate answer from the options given below:

Both Statement I and Statement II are correct

Both Statement I and Statement II are incorrect

Statement I is correct but Statement II is incorrect

Statement I is incorrect but Statement II is correct

In the following palindromic base sequences of DNA, which one can be cut easily by particular restriction enzyme?

5 ' G A A T T C 3 ' 3 ' C T T A A G 5 '

5 ' C T C A G T 3 ' 3 ' G A G T C A 5 '

5 ' G T A T T C 3 ' 3 ' C A T A A G 5 '

5 ' G A T A C T 3 ' 3 ' C T A T G A 5 '

Which one of the following statement is not true regarding gel electrophoresis technique?

The presence of chromogenic substrate gives blue coloured DNA bands on the gel.

The separated DNA fragments are stained by using ethidium bromide.

Bright orange coloured bands of DNA can be observed in the gel when exposed to UV light.

The process of extraction of separated DNA strands from gel is called elution.

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): Polymerase chain reaction is used in DNA amplification. Reason (R): The ampicillin-resistant gene is used as a selectable marker to check the transformation. In light of the above statements, choose the correct answer from the options given below :

Both (A) and (R) are correct but (R) is not the correct explanation of (A)

(A) is correct but (R) is not correct

(A) is not correct but (R) is correct

Both (A) and (R) are correct and (R) is the correct explanation of (A)

EASY

NEET

IMPORTANT

Earn 100

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

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Important Questions on Biotechnology - Principles and Processes

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET - 12 September 2021>Q 10

During the purification process for recombinant DNA technology, addition of chilled ethanol precipitates out:

HARD

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET - 12 September 2021>Q 11

Plasmid

pBR 322

has PstI restriction enzyme site within gene

{amp}^{R}

that confers ampicillin resistance. If this enzyme is used for inserting a gene for

β

-galactoside production and the recombinant plasmid is inserted in an E. coli strain

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET - 12 September 2021>Q 12

During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET - 12 September 2021>Q 13

A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is:

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET Phase 2 (14 October 2020)>Q 14

First discovered restriction endonuclease that always cuts

DNA

molecule at a particular point by recognising a specific sequence of six base pairs is:

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET Phase 2 (14 October 2020)>Q 15

In Recombinant $DNA$ technology antibiotics are used:

*Note: There was ambiguity in options in the original paper, hence the options are changed.

MEDIUM

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET Phase 2 (14 October 2020)>Q 16

Match the following techniques or instruments with their usage:

(a)	Bioreactor	(i)	Separation of DNA fragments
(b)	Electrophoresis	(ii)	Production of large quantities of products
(c)	$PCR$	(iii)	Detection of pathogen, based on antigen-antibody reaction
(d)	$ELISA$	(iv)	Amplification of nucleic acids

Select the correct option from following:

EASY

NEET

IMPORTANT

EMBIBE CHAPTER WISE PREVIOUS YEAR PAPERS FOR BIOLOGY>Chapter 33 - Biotechnology - Principles and Processes>NEET Phase 2 (14 October 2020)>Q 17

In a mixture,

DNA

fragments are separated by:

Which of the following is not an application of PCR (Polymerase Chain Reaction)?

Important Questions on Biotechnology - Principles and Processes

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