Recombinant DNA Technology

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Recombinant DNA Technology: Overview

This topic covers concepts, such as, Recombinant DNA Technology,Isolation of Genetic Material,Tools of Recombinant DNA Technology etc.

Important Questions on Recombinant DNA Technology

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Which statement is correct for bacterial transduction?

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Transfer of genetic information from one bacterium to another in the transduction process is through –

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What is the purpose of transfection?

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What is the purpose of a transfection?

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What is the purpose of transfection?

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Why recombinant DNA technology is used?

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What is Transfection?

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Which ONE of the following statements is INCORRECT about restriction endonucleases?

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Which of the following tools are required for preparation of a recombinant DNA molecule?

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Transduction process is discovered in the bacterium_____by Zinder and Lederberg.

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Transduction is a mode of genetic transfer from one bacterium to another through a fungus.

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Fill in the blank with the correct option provided in the bracket.

_____ (Z-DNA/Plasmid DNA/Recombinant DNA/Passenger DNA) is a modified DNA that is produced by the addition of a foreign DNA into the basic DNA of any organism.

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Which of the following tools are required for preparation of a recombinant DNA molecule?

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Recombinant DNA technology was developed in the early

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A bacteriophage is a _____ that infects bacteria.

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The cell or organism from which the required gene for rDNA technique is taken is called recipient.

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Study the following statements.
A) In germ-line gene therapy functional genes are introduced into somatic cells.
B) Synthetic oligo deoxy nucleosides are used for silencing the disease-causing genes.
C) The transgenic animal product α -lactalbumin is used to treat emphysema.
D) Viral vectors are used to send genetically modified DNA by a process called transfection.

The correct statements are:

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Choose the correct statements.

A. Early detection of pathogen presence can be done by PCR.

B. Biochemical products produced by bacteria are inferior to products produced in transgenic plants.

C. Validity of the GM Research will be controlled by the Genetic Engineering Approval Committee.

D. Use of bioresources by multinational companies with proper authorization is not possible.

The correct answer is-

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Key tools of r-DNA technology are
I. Molecular scissors
II. PBR 322
III. Gene gun
IV. Bioreactor

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Arrange the processes of recombination DNA technology in a sequence.
A) Fragmentation of DNA
B) Transfer of rDNA to host
C) Isolation of desired DNA fragment
D) Isolation of DNA
E) Culturing of host cells and extraction
F) Ligation of DNA into vector