Biotechnological Applications in Medicine
Important Questions on Biotechnological Applications in Medicine
is an enzyme which is deficient in a genetic disorder What is the full form of
The first clinical gene therapy was done for the treatment of
peptide of human insulin is
Which of the following types of ELISA contain the following steps?
Antigen binding, Blocking, Primary antibody, Secondary antibody, Enzyme-linked antibody, Substrate, Colorimetric reading (Represented in diagram).
Select the incorrectly matched pair.
| (i) | Monoclonal antibodies | Hybridomas |
| (ii) | PCR | Phenylketonuria |
| (iii) | Bioweapons | Bacillus anthracis |
| (iv) | Tracy | First transgenic animal for food production |
Select the correct options to fill up the blanks.
(i) _____ enzyme is crucial for the immune system to function and its absence is caused by the deletion of a gene.
(ii) Insulin consists of _____ and _____ that are linked together by _____.
(iii) Transgenic mice are being used to test the safety of the _____.
(iv) _____ involves silencing of a specific mRNA due to a complementary dsRNA molecule that binds to and prevents translation of the mRNA.
Match column I with column II and select the correct option from the given codes.
| column I | column II | ||
|---|---|---|---|
| -1-antitrypsin | AIDS | ||
| Transposon | Gene therapy | ||
| ELISA | Emphysema | ||
| Retroviral vector | Mobile genetic element |
Second generation vaccines are prepared by recombinant DNA technology. Which out of the following are the examples of such vaccines?
Molecular probes are used for many genetic disorders like
Read the following statements regarding ELISA and select the incorrect one.
A technique is used to detect the DNA by _______________, after the separation of DNA by Gel electrophoresis.
_____ is a single-stranded DNA or RNA, tagged with a radioactive molecule and is used to detect mutated genes.
A doctor while operating on an HIV (+)ve patient accidentally cuts himself with a scalpel. Suspecting himself to have contracted the virus which test will he take to rule out/confirm his suspicion?
Which of the following statements is not correct?
Read the given statements and select the correct option.
Statement 1: PCR technique is helpful in detecting bacterial and viral diseases even when symptoms of the disease are not yet visible.
Statement 2: Very low concentrations of bacteria or viruses in human body can be detected by amplification of their nucleic acids using the PCR technique.
Early detection of a disease is possible by
For effective treatment of a disease
Figure given below depict the procedure for gene therapy. Pick up the disorders for which this technique has been applied successfully.
Read the following steps which are followed during the process of gene therapy while treating a patient of SCID. Arrange these steps in an order.
(i) Retrovirus infects lymphocytes extracted from bone marrow of the patient and cultured.
(ii) Engineered cells are injected into patient's bone marrow.
(iii) Normal allele is inserted into a retrovirus.
(iv) Retrovirus makes a DNA copy of its RNA. This DNA carrying the normal allele gets inserted into the chromosome of the host cell.
Arrange the above given steps in correct sequence and select the correct option.
What might be an advantage of beginning gene therapy prior to birth?
