Enzymes
Enzymes: Overview
This topic describes enzymes. It describes the ideas about some chemical reactions which occurs during formation of enzymes, their nature and all factors which are affecting enzyme activities.
Important Questions on Enzymes
Study the given data and answer the following question.
A sample of an enzyme called lactase was isolated from the intestinal lining of a calf. Assays were undertaken to evaluate the activity of the enzyme sample. The substrate of lactase is the disaccharide lactose. Lactase breaks a lactose molecule in two, producing a glucose molecule and a galactose molecule.
Two assays were carried out.
Assay 1:
| Lactose concentration (% w/v) | 15 | 15 | 15 | 15 | 15 | 15 |
| Concentration of enzyme sample (% v/v) | 0 | 5 | 10 | 15 | 20 | 25 |
| Reaction rate glucose sec-1 mL-1 | 0 | 25 | 50 | 75 | 100 | 125 |
Assay 2:
| Lactose concentration (% w/v) | 0 | 5 | 15 | 20 | 25 | 30 |
| Concentration of enzyme sample (% v/v) | 5 | 5 | 5 | 5 | 5 | 5 |
| Reaction rate glucose sec-1 mL-1 | 0 | 15 | 25 | 35 | 40 | 40 |
Which of the following statements can be concluded from the two assays?
Mention difference between Inorganic catalyst and enzymes.
The enzyme DNA ligase is also known as molecular scissor.
Which among the following enzymes, help in joining the molecules together?
Citrate synthase and Pyruvate carboxylase are the two examples of which class of enzymes?
The enzyme that transfers any ion or group except hydrogen from the substrate to any other substrate belongs to the _____ class of enzymes.
Which of the following statement(s) is true about enzymes?
An organic substance bound to an enzyme and essential for its activity is called _____. (coenzyme/cofactor)
An enzyme that catalyzes the conversion of an aldose sugar to a ketose sugar would be classified as one of the _____.
Name the class of enzymes that break chemical bonds when water is added.
Due to _____ nature of the enzymes, they remain active in both acidic and basic medium.
Enzymes that catalyse interconversion of optical, geometrical or positional isomers are
