Transcription

Monocistronic gene is also found in prokaryotes.

Define monocistronic gene.

In what types of cells can you find monocistronic genes?

Briefly explain the process of termination of transcription in eukaryotes.

_____ factor is responsible for the process of transcription to take place in prokaryotes.

Briefly describe the process of termination of transcription in prokaryotes.

A termination sequence is a DNA sequence located towards the 3′ end (downstream) of the coding strand that provides the stop signal and ends the process of transcription.

Which ONE of the following polymerases transcribes $5\mathrm{S} \mathrm{rRNA}?$

Which type of RNA polymerase enzyme transcribes precursor mRNA?

Which type of RNA polymerase enzyme transcribes precursor mRNA? 

In 1958 Francis _____ proposed that DNA determines the sequence of amino acids in a polypeptide through mRNA, this is the main principle or central dogma of protein synthesis. (Write the last part of the name)

DNA$\stackrel{\mathit{X}}{\to }$ mRNA $\stackrel{\mathit{T}\mathit{r}\mathit{a}\mathit{n}\mathit{s}\mathit{l}\mathit{a}\mathit{t}\mathit{i}\mathit{o}\mathit{n}}{\to }$Protein

Identify the process"X" in the given flow chart.

Find out the series of genes in the transcription unit of the following statements.

I. Located towards 3 '-end of the structural gene.

II.  Flanked by the promoter and the terminator.

III. Located towards 3'-end of the coding strand.

Match the following

The correct answer is

Assertion (A) : During transcription both the strands of DNA are copied

Reason $\left(\mathrm{R}\right):$ If both strands act as template DNA would code for RNA molecule with different sequences

Assertion (A): The structural gene in a transcription unit can be said to be monocistronic or polycistronic.

Reason (R): In eukaryotes, the monocistronic structural gene has interrupted coding sequence - but the genes in eukaryotes split. The coding sequence or expressed sequences are defined as exons. Exons are said to be those sequence that appears in mature or processed $\mathrm{RNA}.$
The correct answer is

What is the corresponding sequence of $\mathrm{mRNA}$ when a polynucleotide template $\mathrm{DNA}$ strand is $3^{\text{'}}-\mathrm{TAC} \mathrm{GGT} \mathrm{AAG} \mathrm{CGG} \mathrm{ATC}-5^{\text{'}}$?

Read the following statements and choose the correct option.
(i) Tailing: It is addition of adenylate residues at 5' -end in a template independent manner.
(ii) Promoter: It is the site recognised by the RNA polymerase in association with $\rho$ factor.
(iii) Division of labour: RNA polymerase I and RNA polymerase III are responsible for 18S rRNA and tRNA transcription respectively.
(iv) Capping: addition of methyl guanosine triphosphate at the 5^'-end of hnRNA.

If the template strand of DNA is $3\text{'}\mathrm{ATGCATGCATGCATGC} 5\text{'},$ identify the sequence of RNA transcribed.