Tools for Genetic Engineering
Tools for Genetic Engineering: Overview
This Topic covers sub-topics such as Alkaline Phosphatase, Restriction Enzymes, Bacterial Artificial Chromosome, Cloning Vectors, Transposons, Ti Plasmid, Plasmid as Vector, Expression Vectors, Shuttle Vectors and, Tools of Recombinant DNA Technology
Important Questions on Tools for Genetic Engineering
Explain shuttle vectors.
Define phagemid vectors.
Define expression vectors.
Describe the significance of Ti plasmid.
Name the marker sites of pBR322 plasmid.
Define cloning vector.
Mention one function of alkaline phosphatase.
Which of the following is used as the host in the vector YAC?
_____ (BAC/YAC) vector uses Yeast as host.
Define YAC.
The commonly used host for the DNA sequencing in vector YAC is Yeast.
_____ (BAC/YAC) vector uses bacteria as host.
Which of the following is used as the host in the vector BAC?
Define BAC.
The commonly used host for the DNA sequencing in vector BAC is virus.
The normal E. coli cells do not carry resistance against
Cosmid is a hybrid of plasmid and lambda _____
The viruses that infect bacteria are called _____ and they are used as vector in genetic engineering.
Fill in the blank with the correct option from the bracket.
The category of the transposon which can perform reverse transcription is _____. (class II transposons, miniature inverted-repeat transposons, retrotransposons)
The common form of _____ or jumping gene in humans is called the _____ sequence.
